Antibiotic Resistance Among Klebsiella pneumoniae, Molecular Detection and Expression Level of blaKPC and blaGES Genes by Real-Time PCR
نویسندگان
چکیده
منابع مشابه
Rapid detection of blaKPC carbapenemase genes by real-time PCR.
Carbapenem resistance among Enterobacteriaceae is an emerging problem worldwide. Klebsiella pneumoniae carbapenemase (bla(KPC)) enzymes are among the most common beta-lactamases described. In this study, we report the development and validation of a real-time PCR (q-PCR) assay for the detection of bla(KPC) genes using TaqMan chemistry. The q-PCR amplification of bla(KPC) DNA was linear over 7 l...
متن کاملبررسی مقاومت آنتی بیوتیکی ایزوله های کلبسیلاپنومونیه و میزان بیان ژنهای Real-Time PCR بااستفاده ازacrA وoqxA
Background: The increasing emergence of antimicrobial resistance among gram negative bacteria especially Enterobacteriaceae has become a global problem. Klebsiella pneumoniae is an opportunistic pathogen, which recently due to causing broad spectrum of disease and antibiotic resistance has been lionized. Efflux pumps are one of the antibiotic resistance mechanisms, which were introduced in t...
متن کاملRapid and sensitive detection of blaKPC gene in clinical isolates of Klebsiella pneumoniae by a molecular real-time assay
BACKGROUND The aim of this study was the rapid identification of bla KPC gene in 38 Klebsiella pneumoniae clinical isolates with reduced susceptibility to carbapenems. The modified Hodge Test (MHT) was carried out to phenotypically determine whether resistance to carbapenems was mediated by a carbapenemase. The detection of the bla KPC gene was performed by real-time acid nucleic sequence-based...
متن کاملDevelopment and evaluation of a real-time PCR assay for detection of Klebsiella pneumoniae carbapenemase genes.
We developed a novel real-time PCR assay to detect Klebsiella pneumoniae carbapenemases (KPCs) and used this assay to screen clinical isolates of K. pneumoniae and Klebsiella oxytoca for the presence of bla(KPC) genes. The TaqMan real-time PCR assay amplified a 399-bp product from the bla(KPC) gene. The amplicon was designed so that the genes for isoenzymes KPC-1, -2, and -3 could be easily dis...
متن کاملReal-time RT PCR Evaluation of the Xylitol Effect on the Expression of Streptococcus pneumoniae cpsB,cpsD and psaA Genes
Background and Objective: The major pneumococcal pathogenesis factor is the capsular polysaccharide. the production of polysaccharide is regulated by cpsB and cpsD genes. Thus, every agent that induce or inhibition of expression of these genes probably increased pathogenesis of bacteria, intracellular survival and vis versa. The aim of this study was to assay the effect of Xylitol on Streptococ...
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ژورنال
عنوان ژورنال: Jundishapur Journal of Microbiology
سال: 2019
ISSN: 2008-3645,2008-4161
DOI: 10.5812/jjm.93070